I am considering making some yeast slants to try to start a little bit of a yeast bank. In my research i have read about folks making a starter from the yeast in the bottom of a bottle conditioned beer, then inoculating the slants from that. I have also read about washing the yeast cake, but cant seem to find if there is another step between washing the yeast and inoculating slants. Is it better to just wait and use the yeast in the bottom of the bottles, or use the washed yeast cake.
It seems to me that the yeast in the carboy might be a healthier sample having not been in the alcohol as long, but there would also be a lot more unwanted material. If this is the best route, is it necessary to harvest from a starter from the washed yeast, or can i inoculate the slants straight from the washed yeast, or do i need to wash it at all.
The yeast in the bottles would be cleaner, but might have more mutations etc.