I am preparing to start my own small yeast bank and I was wondering what is the optimal method for scaling up to a starter from a single colony.
- What step sizes should I use?
- What strength of wort should I use?
- How long should it take for each step?
For the sake of an example, what would be the optimal path to get from a single colony on a plate to a starter suitable for pitching into 100l of 1050 wort?
Edit for clarity: By optimal I mean giving me the smallest pitchable starter, for the required number of cells, and having cells at the highest viability and vitality when pitching.