I attempted my first yeast washing last night after bottling. I added 1 quart of boiled-then-cooled water to my carboy, swished the yeast cake into suspension, then filled 2 quart jars, each about 3/4th's full. After about 30 minutes there was maybe 1/2" of seperation. At this time, I really needed to get to bed so I let it sit overnight. In the morning (about 7 hours later) there was a lot more seperation, but the top layer was much clearer than any of the videos I've watched online.
My question is, is it possible too much of the yeast fell out of suspension? Also, I'm confused if there is anything else that needs to be in the jars besides water and yeast cake. Does it need nutrients?